Pt. 1. Key concepts for PCR-- Ch. 1. Optimization of PCR: conversations between Michael and David-- Ch. 2. The convergence of PCR, computers, and the human genome project: past, present, and future-- ...

Here, we describe a method of systematic PCR screening with multiround sample pooling for the isolation of rare PCR-positive samples. As an example, we have applied this protocol to the recovery of ...

Young scientists often get frustrated with laborious experimental procedures, such as cloning, PCR, or sequencing. However, the Internet has made planning and performing these procedures faster and ...

My cry of frustration echoed down the hall. My latest attempt to subclone five important PCR products had failed yet again. After two months of fruitless effort, I was at my wit's end until a ...

Colony picking is a very labor-intensive task that is generally performed during cloning protocols, alongside additional upstream and downstream processes. Manual colony picking is both slow and ...

This is a preview. Log in through your library . Abstract Cellulose biodegradation is based upon the action of different microbial enzymes such as β-1,4-endoglucanases, exoglucanases, and ...